Cytosolic Selection Systems To Study Protein Stability

Cytosolic selection systems to study protein stability.

Here we describe biosensors that provide readouts for protein stability in the cytosolic compartment of prokaryotes. These biosensors consist of tripartite sandwich fusions that link the in vitro stability or aggregation susceptibility of guest proteins to the in vivo resistance of host cells to the antibiotics kanamycin, spectinomycin, and nourseothricin. These selectable markers confer antibi...

Selection maintaining protein stability at equilibrium.

The common understanding of protein evolution has been that neutral mutations are fixed by random drift, and a proportion of neutral mutations depending on the strength of structural and functional constraints primarily determines evolutionary rate. Recently it was indicated that fitness costs due to misfolded proteins are a determinant of evolutionary rate and selection originating in protein ...

Evolving protein stability through genetic selection.

In this issue of Molecular Cell, Foit et al. (2009) probe cellular protein folding using a split beta-lactamase approach for evolving protein stability in the absence of any requirement for function.

Estimating stability and selection index

Stability selection

Estimation of structure, such as in variable selection, graphical modelling or cluster analysis is notoriously difficult, especially for high-dimensional data. We introduce stability selection. It is based on subsampling in combination with (high-dimensional) selection algorithms. As such, the method is extremely general and has a very wide range of applicability. Stability selection provides f...